Rapid solubility test for detection of hemoglobin S.

False positive results with a rapid solubility test for haemoglobin S.

Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT

Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 ...

A rapid whole blood solubility test to differentiate the sickle-cell trait from sickle-cell anaemia.

A simple and rapid screening test which differentiates sickle-cell trait and sickle-cell anaemia is described. The test utilizes 0.1 ml of whole blood and is based on the low solubility of reduced sickle haemoglobin. Results intermediate between the sickle-cell trait and sickle-cell anaemia are obtained in unusual cases of sickle-cell anaemia with high foetal haemoglobin. The need to supplement...

Detection of Infectious Bronchitis Virus in Allantioc Fluid by Rapid Hemagglutination Test.

To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by...

A modification of the "Sickledex" test for hemoglobin S.

The “Sickledex” test (as described by Diggs et al., in a scientific exhibit, 1968, and marketed by Ortho Diagnostics, Raritan, N. J. 08869) has gained widespread use as a screening method for hemoglobin S (1, 2). Apparently based on the solubility test of Itano (3), it has been shown to be positive with abnormal hemoglobins other than the S hemoglobin (4). Nevertheless, it is a quick, simple sc...